NAD⁺ (500MG) Analytical Reference Material (In Vitro Research Only)
NAD⁺ (nicotinamide adenine dinucleotide) is a small molecule found in every living cell. It helps cells turn nutrients into energy and is a required partner for enzymes such as sirtuins and PARPs. In the lab it is used as a substrate in enzyme assays and cell-metabolism studies.
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1:1 Analytical & Chemical Specifications
| Analytical Parameter | Verified Specification |
|---|---|
| CAS Number | 53-84-9 |
| Molecular Formula | C21H27N7O14P2 |
| Molecular Weight | 663.43 g/mol (Da) |
| Amino Acid Sequence | Nicotinamide Adenine Dinucleotide (Dinucleotide / non-peptide) |
| Chromatographic Purity (RP-HPLC) | >=99.0% Peak Area (Observed Lot 75442: 99.6%) |
| Identity Verification | Electrospray Ionization Mass Spectrometry (ESI-MS) |
| Physical Form | Lyophilized Powder in Sealed Desiccated Glass Vial |
| Recommended Storage | -20°C Desiccated & Protected from Light |
| Regulatory Classification | Research Use Only (RUO) — Not a 503A Compounding Pharmacy Product; Strictly Not for Human or Veterinary Use |
| Lot Traceability | Lot 75442 (Tested 2026-01-22) — Verify Dataset in COA Library → |
Lot-Specific Certificate of Analysis (COA)
In Vitro Laboratory Research Scope
Typical laboratory uses: sirtuin deacetylase assays, PARP DNA-repair activity assays, and studies of mitochondrial redox (energy-transfer) reactions. For in-vitro laboratory research only. Not for human or animal use.
Analytical Verification & Storage Parameters for NAD⁺
How is the chromatographic purity of NAD⁺ (500MG) verified?
Every production batch of NAD⁺ (500MG), including active Lot 75442, undergoes independent Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) with UV/Vis detection at 214 nm and Electrospray Ionization Mass Spectrometry (ESI-MS), confirming 99.6% peak area purity (retention time 4.62 min) and exact molecular mass concordance prior to catalog release.
What are the laboratory storage and stability parameters for lyophilized NAD⁺?
Lyophilized NAD⁺ reference material is supplied in a sealed, desiccated borosilicate glass vial under inert atmosphere and must be stored at -20°C protected from light to prevent hydrolytic or oxidative cleavage. Once solubilized in sterile aqueous buffer for in vitro assays, maintain aliquots at 2°C to 8°C and avoid repeated freeze-thaw cycles.
What are the chemical identifiers and regulatory classification of NAD⁺?
NAD⁺ is characterized by CAS Registry identifier 53-84-9, molecular formula C21H27N7O14P2, and theoretical molecular weight 663.43 g/mol (Da). Regenerative Health Peptides supplies this reagent strictly as a Research Use Only (RUO) analytical reference material for qualified 21+ in vitro laboratory investigations, never for human or veterinary use.
Primary Scientific Literature & Preclinical Citations
Peer-ReviewedThe following peer-reviewed scientific publications report preclinical investigations into the biochemical mechanisms and receptor pharmacology of this compound. Regenerative Health Peptides supplies this material strictly for in-vitro laboratory research.
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