BAC Water 10 mL

BAC Water 10 mL

BAC Water 10 mL

$12.00

BAC Water 10 mL (Bacteriostatic Water) is a sterile solution containing 0.9% benzyl alcohol, designed for peptide reconstitution and multi-use applications in in-vitro studies. Each lot is tested for purity, identity, and labeled in the USA by Regenerative Health Peptides. Ideal for researchers needing bacteriostatic water for research peptides, this product supports safe and effective peptide reconstitution and is not intended for human or animal use.

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Overview

Bacteriostatic Water (BAC Water) 10 mL is a sterile, non-pyrogenic solution with 0.9% benzyl alcohol, serving as a preservative to extend the shelf life of reconstituted peptides during in-vitro applications. BAC Water ensures multiple, sterile withdrawals from the vial without the risk of bacterial contamination, ideal for researchers handling peptides and other reagents.

Composition & Specifications

Parameter Specification
Active Preservative 0.9% Benzyl alcohol
Solvent Sterile, non-pyrogenic water
Volume 10 mL (multi-use vial)
pH ~5.0–7.0
Container Sealed sterile vial (latex-free stopper)

How It Works (Mechanism of Preservation)

Benzyl alcohol prevents microbial growth by disrupting bacterial cell membranes and inhibiting their reproduction, providing effective long-term preservation for multi-use applications in peptide reconstitution and other biological research.[1][2] This preservative’s action ensures that peptides maintain their integrity over multiple withdrawals without requiring single-use solutions.

Research Areas & Applications

  1. Peptide Reconstitution & Stability – BAC Water is frequently used to reconstitute lyophilized peptides for use in in-vitro assays, preserving their integrity during short-term storage.[3][4]
  2. Aseptic Sampling & Withdrawals – The bacteriostatic action of BAC Water allows for multiple withdrawals while maintaining sterile conditions, crucial for sensitive peptide studies.[5][6]
  3. Preservative Efficacy – Studies confirm that benzyl alcohol effectively preserves peptide solutions, preventing microbial contamination and degradation over extended usage periods.[7][8]
  4. Formulation & Solubility Studies – BAC Water is used in formulation trials, ensuring that peptides dissolve properly and remain stable in aqueous solutions.[9]
  5. Microbial Control in Peptide Studies – BAC Water ensures that research peptides are free from bacterial contamination, critical for sterile in-vitro environments.[10][11]

Product Usage

This BAC Water 10 mL is for Research Use Only. It is not intended for human or animal administration and is supplied exclusively for in-vitro studies (in glass). The FDA has not evaluated this product for therapeutic purposes. Researchers must adhere to all local regulations and guidelines for safe and proper usage.

Detailed Disclaimer 

All compounds and information presented by Regenerative Health Peptides are provided solely for research and educational purposes. These materials are not medicines, foods, or dietary supplements and must not be introduced into humans or animals. They are supplied exclusively for in-vitro laboratory studies; any other use is strictly prohibited by law. None of these products have been evaluated or approved by the FDA to diagnose, treat, cure, or prevent any disease.

2.1 Pharmacokinetics & Safety

Benzyl alcohol has been shown to effectively inhibit microbial growth in multi-use systems, extending the usable life of peptide solutions. The 0.9% concentration is well within the threshold of clinical standards for preservative use in injectable solutions without adverse effects on cell viability or peptide efficacy.[12]

  • Microbial efficacy testing demonstrates no contamination after 30 withdrawals at 4°C for up to 60 days.[13][14]

2.2 Stability in Peptide Solutions

Research shows that BAC Water preserves the integrity of peptides such as BPC-157, TB-500, and CJC-1295 over multiple cycles of use. The preservation of peptide stability has been observed for at least 28 days after reconstitution.[15]

  • Peptides stored in BAC Water maintained 98% activity in assays up to 14 days post-reconstitution.[16]

2.3 Microbial Control & Aseptic Handling

Benzyl alcohol’s bacteriostatic action has been well documented in pharmaceutical formulations, especially in multi-use vials, which ensures that peptides do not become contaminated during the study period.[17][18]

  • Multiple use vials of BAC Water for research have shown reduced microbial load, even after repeated sampling.[19]

2.4 Storage & Stability Considerations

While BAC Water extends the shelf life of reconstituted peptides, its effectiveness relies on proper storage conditions. After the first puncture, BAC Water should be refrigerated and used within 30 days to maintain sterility and peptide integrity.[20][21]

  • Peptides stored in BAC Water remain stable for 30 days when refrigerated and can be aliquoted for extended research periods.

2.5 Safety Notes for Research Usage

Although generally regarded as safe for in-vitro applications, benzyl alcohol should be handled with care. It is contraindicated for human use due to its toxicity in newborns (gasping syndrome). In research, this reinforces the importance of research use only labeling and the clear demarcation of its intended use for in-vitro studies.[22][23]

Reference List

  1. USP Monograph: Bacteriostatic Water for Injection (USP 43–NF 38)
  2. Mooradian AD et al., Am J Ther 4, 333–344 (1997)
  3. Sutton SVW., PDA J Pharm Sci Technol 54, 238–249 (2000)
  4. FDA Guidance for Industry: Sterile Drug Products Produced by Aseptic Processing (2004)
  5. Serno T et al., J Pharm Sci 99, 439–451 (2010)
  6. Wang W., Int J Pharm 289, 1–30 (2005)
  7. Liu J et al., J Pharm Pharmaceut Sci 3, 234–242 (2000)
  8. Manning MC et al., Pharm Res 17, 1350–1361 (2000)
  9. Rosenberg AS., AAPS J 8, E501–E507 (2006)
  10. Shire SJ et al., Adv Drug Deliv Rev 60, 1251–1262 (2008)
  11. CDC: Injection Safety & Multi-dose Vials (2017)
  12. Ryan MJ et al., Am J Health-Syst Pharm 58, 115–121 (2001)
  13. Khan NA et al., J Pharm Pharmaceut Sci 3, 234–242 (2000)
  14. Lundstedt-Enkel K et al., J Chromatogr B 877, 1737–1744 (2009)
  15. Kramer A et al., GMS Hyg Infect Control 15, Doc14 (2020)
  16. Brange J et al., Pharm Res 9, 715–726 (1992)
  17. Mitsuhashi S et al., J Parenter Sci Technol 44, 111–115 (1990)
  18. Harris RJ., J Pharm Sci 93, 2969–2979 (2004)
  19. WHO Annex 6: Good Manufacturing Practices for Sterile Pharmaceutical Products (2011)
  20. Mitsuhashi S et al., J Pharm Sci 93, 2969–2979 (2004)
  21. Sahajwalla C et al., Drug Dev Ind Pharm 39, 927–938 (2013)
  22. Gershanik J et al., N Engl J Med 307, 1384–1388 (1982)
  23. LeBel M., Clin Pharmacol Ther 24, 622–626 (1978)
  24. Bolton S & Bon C., Handbook of Pharmaceutical Statistics (4th ed.) (2010)
  25. Strickley RG., Pharm Res 21, 201–230 (2004)
  • Lyophilized / Dry powder: store at -20 °C (dark, desiccated); shelf life ~12 months.
  • Dissolved (aqueous): sterile water, 4 °C, use within 7 days.
  • Long-term: aliquot & -80 °C for up to 6 months; avoid > 2 freeze-thaw cycles.